10. Take a sample of cell suspension and mix with 0.1% Trypan

blue solution at 1:1 ratio. Count cells to calculate the total cell

number in the culture vessel and cell viability (see Note 13,

Figs. 6 and 7).

Fig. 6 Bright field images of microcarrier dissolution process, with microcarriers been fully dissolved by

30 min and all cells were released

Fig. 7 Cell growth curve of hUCMSCs on 3D TableTrix^® microcarriers in 3D FloTrix^® vivaSPIN bioreactor. Cell

number on day 0 is the initial seeding number. Numbers of cells for days 1–3 are calculated based on

sampling, and for day 4 is based on the total number of cells harvested. All cell numbers reported are total live

cell numbers

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